Chapter 64 – Finally broke through the fifth generation!

Four days later.

Biomedical laboratory.

At this moment.

The entire laboratory was filled with a tense atmosphere mixed with a hint of excitement. Members of the research group wearing lab coats were constantly walking back and forth.

Not to mention Tang Yiqiu, who was originally lively, even Ren Yongcun, a somewhat introverted and shy first-year graduate student, occasionally showed a look of anticipation in his eyes.

In fact, the reason for the intense emotional fluctuations of these few "tools" was very simple:

After several days of working overtime, the interference protein inducer that Xu Yun had previously conceived, which could attract male cockroaches to interact with each other, had been successfully created!

Now, the research task of the entire research group had reached the final and most critical stage:

To synthesize the inducer and the fourth-generation pyrrolidine derived by Xu Yun and test whether it could act on the MC38 cell.

MC38 cells were one of the cutting-edge cells in current biological research.

They originated from the C57BL/6 mouse colon adenocarcinoma cell line, grew adherently, and had fibroblast-like morphology.

When MC38 cells were implanted into C57BL/6 mice or immunodeficient mice, tumors and metastases would form. Therefore, this technique was often used for research on the occurrence and metastasis of colorectal cancer and became one of the common methods for validating tumor efficacy.

However, these cells did not belong exclusively to mice. They were also abundant in cockroaches, dragonflies, and mantises.

They were a type of cell that had more research information in the field of gene editing.

And this was exactly why Xu Yun had targeted this anchor point:

Firstly, it was related to the sodium ion channels of cockroaches and was an important electrical signaling site for sodium ions.

Secondly, there were many authoritative experimental results in various fields of biology, so there was no need to spend a lot of time and effort to deduce and study.

While Tang Yiqiu and the others were busy outside, Xu Yun and Qiu Sheng were standing in the operating room, making final preparations for the practical operation:

"Qiu, are you sure you can handle this?

Let me tell you, this round of experiments has no room for error. If anything goes wrong, we'll have to start over from scratch. You can't even shake your hands a bit—by the way, you didn't stay up late last night, did you?"

"Don't worry, I didn't have time to watch the Yamate Riai series last week... Ah, what did you say?"

Qiu Sheng, who had been concentrating on adjusting the equipment, subconsciously replied with half a sentence and suddenly felt that something was wrong. He glared at Xu Yun irritably:

It can be hard to make great work when its stolen from "pawread dot com".

"Come on, why don't you do it, and I'll take notes on the side?"

Seeing this, Xu Yun chuckled. He and Qiu Sheng were childhood friends, so he naturally knew whether the other party was really angry or not.

As the saying goes, everyone has their own expertise. Qiu Sheng was recognized as the number one person among the doctoral students at the University of Science and Technology in the field of guided group-assisted synthesis, while Xu Yun's advantage was not in this direction.

The reason why Xu Yun asked Qiu Sheng to help was mostly because of the current stage of the experiment, so it was impossible to change personnel:

"Just kidding, just kidding. Hurry up and adjust the equipment, let's get started as soon as possible.

By the way, send me the latest episode of the Yamate Riai series later. I have a friend called Reader who wants to take a look."

"..."

The term "material synthesis" may not be unfamiliar to many people. Their ears have almost become calloused from hearing it.

But in reality, most people's understanding of this term only scratches the surface and is not in-depth.

For example, what does a biological laboratory look like? What is the process of synthesis like?

If you really want a detailed description of the steps in words, I'm afraid that less than one percent of people would know.

In general, material synthesis can be divided into two concepts:

Known material synthesis and unknown material synthesis.

The operation of the former is very simple. It only requires forward synthesis analysis and reverse synthesis analysis to find intermediates, which is high school knowledge.

As for the synthesis of unknown substances, it is more complicated.

If the synthesis of unknown substances involves biological substances, it will inevitably involve polymerase chain reaction.

Through this reaction, DNA molecules, which are originally in extremely small amounts, can be amplified tens of thousands to millions of times.

Then, through design, it can be used to extract the required DNA information from small amounts of biological samples, achieving sensitivity at the level of single cells or even single molecules.

At the same time, specific primers can be designed to combine different DNA fragments to synthesize new DNA molecules.

The method used by Xu Yun and the others this time was solid-phase chemical synthesis, which used solid substances such as resin as the reaction matrix, with nucleic acids or protein molecules being synthesized on it.

In this way, by continuously filtering and continuously replenishing different raw materials for the reaction, different monomer molecules can be sequentially connected to the matrix to synthesize the desired nucleic acids or proteins.

Twenty minutes later.All preparations were complete, and the synthesis officially began.

Previously, Xu Yun had deduced five types of spatial structures formed by peptide chains, but Qiu Sheng and others only used one of them:

The structure where the C=O and N-H within the peptide plane are arranged in a trans configuration, and the peptide plane rotates 27 degrees around the Cα-N1 bond.

Five minutes later, Xu Yun looked at Qiu Sheng:

"Old Qiu, the biological pathway has been verified, and the target cells have been locked. Shall we proceed to the next step?"

Without lifting his head, Qiu Sheng asked:

"Are the nucleotide monomers protected by DMT?"

"They are."

Qiu Sheng hummed in acknowledgment, not saying anything. He had great faith in Xu Yun.

He then shifted his gaze to a male cockroach fixed in the middle of the operating table, and began editing using the CRISPR/Cas9 technique.

The CRISPR/Cas9 technique was first proposed in 1987. In 2012, two female scientists, Jennifer Doudna and Emmanuelle Charpentier, first discovered the nuclease activity mediated by Cas proteins in the adaptive immune system of bacteria. They were awarded the 2015 Breakthrough of the Year in Life Sciences for this discovery.

Since then, CRISPR/Cas9 has become the most mainstream gene editing system today.

Qiu Sheng locked the binding points calculated by Xu Yun. The target cells were MC38 cells from the cockroach, and the goal was to shatter the directional fragment of feedback gender cognition with hCD47-MC38HuCELL.

Once this fragment was shattered, the cockroach's gender cognition would be affected.

Under the promotion of pheromones, the previously mentioned A+C ketone behavior would occur.

At this moment.

Inside the microscopic world of the unfortunate cockroach.

This was an immensely vast 'planet'. Under the yellow sky, a scene of sin was unfolding:

A spherical pathogen stepped on a piece of cholesterol, tearing it apart with a hiss, and swallowed its broken limbs with a cruel laugh amidst the cholesterol's wailing.

Not far from it, another pathogen held a newly synthesized class I membrane protein high above its head and ruthlessly threw it off a cliff.

Further away, a Coxsackie virus was swinging its double sickles, cutting a sugar variant in half...

Even further away was...

This was a world without any glimmer of hope, without fairness, without hope, without...

The future.

After the slaughter, the pathogen sat at the foot of the mountain, mechanically chewing the cholesterol in its mouth.

It had done this countless times and would continue to do so until it died.

However, just as it was looking for its next target, an anomaly occurred.

At some point, a black dot suddenly appeared in the originally yellow sky.

The black dot was initially very small, but within a mere thousandth of a millisecond, it tore open a huge hole.

Like a torn piece of cloth, it expanded to the farthest reaches of the field of vision.

Before the pathogen could react, something like an anchor bolt suddenly appeared from the hole and rushed towards a fragment floating in the air.

In less than a moment.

The anchor bolt accurately hooked the fragment and pulled it out of this world at a speed faster than when it arrived.

The pathogen, having never seen such a situation, stared dumbfounded at the scene before it.

Just as it was about to call its companions to ask about the situation, a sound like a breaking wind came from its ears.

It instinctively looked up at the sky.

At this moment, countless burning C1galt1c1 inducers were coming from the hole that covered the sky.

They descended on this world with flames and justice.

"Huh?"

The pathogenic organism had just let out an unintelligible cry when it was smashed to pieces by the inducers falling from the sky.

In fact, it wasn't just it.

At this moment.

All the sins of this world were being cleared by the divine punishment from the outside world.

In less than a moment, this sinful world was covered in flames and came to an end.

A few minutes later, in the macro world.

With the series of changes happening in the microscopic realm, the experimental cockroach that was put back into the ecological box suddenly spread its wings.

With a couple of flaps, it darted straight towards a male cockroach...

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